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KMID : 1134820210500040395
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Journal of the Korean Society of Food Science and Nutrition
2021 Volume.50 No. 4 p.395 ~ p.402
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Analytical Method Validation of Cynaroside in Domestic Anthriscus sylvestris (L.) Hoffm. Leaves Extract for Standardization as a Functional Ingredient Using RP-HPLC
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Hong Joon-Ho
Lee Mi-Jin
Jo Young-Ick
Moon Sung-Min
Lee Seul-Ah
Kim Chun-Sung
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Abstract
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This study was undertaken to establish an analytical method using RP-HPLC for standard determination of cynaroside in the leaves extract of domestic Anthriscus sylvestris (L.) Hoffm., as a functional ingredient. The analytical method of cynaroside was optimized by HPLC on a reversed-phase octadecyl-silica (ODS) column with a gradient elution of 0.1% (v/v) trifluoroacetic acid (TFA) and methanol at 40°C and flow rate of 1.0 mL/min. The analyte was detected at 345 nm. The specificity was confirmed by the chromatogram obtained from the HPLC analytical method, as well as the results of UV and ESI-TOF mass spectra, and the coefficient of correlation (R2) obtained was 0.9999, which confirmed this as a suitable analysis with high linearity. The limit of detection (LOD) was 0.26 mg/L, and limit of quantitation (LOQ) was 0.78 mg/L, which was confirmed as a suitable limit level for the analysis of cynaroside content in the Anthriscus sylvestris (L.) Hoffm. leaves extract. The recovery was determined to be 98.55±0.81∼102.53±1.11%, and the relative standard deviation (RSD) was 0.25∼1.09%, indicating high accuracy. The intra-day repeatability and the intra-laboratory precision of the daily repetition were confirmed to be 0.86∼0.91% and 0.81∼1.47%, respectively, for the RSD. These results validate the established HPLC analytical method, by confirming the specificity, linearity, precision, and accuracy to be within the ICH guideline.
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KEYWORD
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Anthriscus sylvestris (L.) Hoffm. leaves, cynaroside, HPLC, analytical method validation, functional ingredient
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